Dissertation defense traditions: Keller group

Submitted by Diana Knight on
A montage of giant unilamellar vesicles for which the lipids in the membrane are either uniformly mixed, or have phase separated into coexisting liquid phases. When the boundary between phases is wavy, the membrane is poised near a miscibility critical point. Microscopy and artwork by Aurelia R. Honerkamp-Smith ‘10.

What are your group’s dissertation defense traditions?

Keller group

Sarah L. Keller, Duane and Barbara LaViolette Endowed Professor of Chemistry:

One year, on a lark, I made a cake that illustrated a graduate student’s research project and used it as a visual aid during my introduction of their dissertation defense. Somehow, the next couple of students’ projects also lent themselves to cakes. (Our lab’s main research focus is on lipid vesicles and cell membranes, which tend to be round. See the stylized montage of microscopy images, above right.) Pretty soon, grad students in my lab began saying, “We are all wondering what your upcoming thesis cake will be!” The pressure was on!

A figure containing four photos of different cakes. The photo on the left is a round yellow cake. The photo at the top of the right column is a yellow cake baking in the over. The middle photo is a yellow cake with three yellow cupcakes at its edges. The bottom photo is a yellow cake decorated with red gummy candies.

Summary of experiments at the Marine Biology Laboratory at Woods Hole in a project led by Chantelle Leveille ‘22. The cake represents a yeast cell that is undergoing changes in temperature (top), is budding (middle), or has fluorescently labeled mitochondria (bottom). See C.L. Leveille et al., 2021, Proc. Natl. Acad. Sci. USA.

A round chocolate cake with white frosting on the top and spherical lollipops bordering the cake's edge, alternating in the up and down positions.

Zack Cohen ’23: The cake is a close-up of a membrane made of fatty acids, which have one carbon chain each, so are represented as lollipops. See Z.R. Cohen et al., 2023, ACS Earth Space Chem.

Sarah Keller holds a rectangular baking pan with a round red cake upon it.

Jonathan Litz ’15: The cake captures the shape and color of a lipid vesicle ruptured on a solid, dark support (and imaged with a Texas red label). See J.P. Litz et al., 2016, Biophys. J.

Sarah Keller holds a round chocolate cake that has a small round white cake embedded in the middle.
A round white cake sits on a board cut in half exposing the interior. The core of the cake is chocolate, but the top of the cake is offset from the base.

Matt Blosser ’14: The top image shows a control sample of a phase-separated membrane with a domain of the bright (“liquid-ordered”) phase surrounded by a dark (“liquid-disordered”) phase. The bottom image is a cut-away view of a test sample of a phase-separated membrane in which the membrane has been sheared to offset the domains. See M.C. Blosser et al., 2015, Biophys. J.

Glennis Rayermann holds a round double layered chocolate cake. The top is frosted white except for 6 circles where the chocolate cake is visible. The frosting has green sprinkles.

Glennis Rayermann ’18: The cake looks like a phase-separated membrane of a yeast vacuole in which contrast is provided by a membrane protein fusion (Vph1-GFP) that emits green light. See S.P. Rayermann et al., 2017, Biophys. J.

A round chocolate cake with a round chocolate brownie embedded in the middle. Atop the brownie is puffed rice cereal affixed by white frosting.

Heidi Weakly ’24: Some proteins (represented by puffed rice cereal) bind to one domain of a phase-separated lipid bilayer (the cookie vs. the surrounding brownie) via molecular tethers (the frosting). See H.M.J. Weakly et al., 2024, Biophys. J.

A round chocolate cake decorated with blueberries around the perimeter of the top.
A dozen chocolate cupcakes each decorated with two blueberries.

Caitlin Cornell ’20: This big round cake has high contrast only at the perimeter because it represents a lipid vesicle imaged by cryo-electron tomography. The technique requires that big vesicles be broken up into smaller (cupcake-sized) vesicles. See C.E. Cornell et al., 2020, Proc. Natl. Acad. Sci. USA.

See also Dissertation defense traditions: Vaughan, Cossairt, and Khalil groups

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