Professor
Fields of Interest
Biography
Ph.D., Massachusetts Institute of Technology, 2005
B.A., Reed College, 2000
Josh grew up in the San Francisco bay area and had an interest in science from an early age. He studied chemistry at Reed College in Portland, OR and earned a Ph.D. in physical chemistry at MIT where he worked in the lab of Keith Nelson on applications of adaptive optics to ultrafast spectroscopy. For his postdoctoral research in Xiaowei Zhuang's lab at Harvard he developed tools for super-resolution fluorescence microscopy and studied virus transport. His current interests include developing new tools to extract molecular detail from intact biological specimens and applying these tools to important biological questions. Outside of work, he enjoys music, sailing, and spoiling his two daughters.
Research
Selected Research
- Halpern, A.R.; Lee, M.Y.; Howard, M.D.; Woodworth, M.A.; Nicovich, P.R.; Vaughan, J.C. Versatile, do-it-yourself, low-cost spinning disk confocal microscope. Biomed. Opt. Express 2022, 13, 1102–1120.
- Woodworth, M.W.; Ng, K.K.H.; Halpern, A.R.; Pease, N.A.; Nguyen, P.H.B.; #Kueh, H.Y.; #Vaughan, J.C. Multiplexed single-cell profiling of chromatin states at genomic loci by expansion microscopy. Nucleic Acids Res. 2021, e82.
- Mao, C.; Lee, M. Y.; Jhan, J.-R.; Halpern, A. R.; Woodworth, M. A.; Glaser, A. K.; Chozinski, T. J.; Shin, L.; Pippin, J.W.; Shankland, S.J.; Liu, J. T. C.; Vaughan, J. C. Feature-Rich Covalent Stains for Super-Resolution and Cleared-Tissue Fluorescence Microscopy. Sci. Adv. 2020, 6, eaba4542.
- Lin, D.; Gagnon, L. A.; Howard, M. D.; Halpern, A. R.; Vaughan, J. C. Extended-Depth 3D Super-Resolution Imaging Using Probe-Refresh STORM. Biophys. J. 2018, 114, 1980–1987.
- Halpern, A. R.; Alas, G. C. M.; Chozinski, T. J.; #Paredez, A. R.; #Vaughan, J. C. Hybrid Structured Illumination Expansion Microscopy Reveals Microbial Cytoskeleton Organization. ACS Nano 2017, 11, 12677–12686. (#co-corresponding authors)
- Chozinski, T. J.; Halpern, A. R.; Okawa, H.; Kim, H.-J.; Tremel, G. J.; Wong, R. O. L.; Vaughan, J. C. Expansion microscopy with conventional antibodies and fluorescent proteins. Nat. Methods 2016, 13, 485–488.
Courses Taught
Winter 2025
Autumn 2024
Spring 2024
Winter 2024
Autumn 2023
Spring 2023
Winter 2023
Autumn 2022
Affiliations
Home Department